To investigate whether targeting BCL-3 expression could increase the sensitivity of colorectal cell lines to gamma irradiation, four high BCL-3 expressing CRC cell lines (colon HCA7, LS174T, LoVo and rectal SW1463,) were transiently transfected with BCL-3 siRNA, seeded in a 6 well plate and irradiated 48 h later with gamma irradiation (1 and 2.5 Gy). Here, BCL3 is linked to colorectal carcinoma.