In this study, mir-181a-5p mimic can significantly reduce the expression, scratch and migration of MMP2/9 Invasion experiments confirmed that mir-181a-5p mimic could inhibit the motility of esophageal cancer cells, and the cells were transfected with MEK1 overexpression lentivirus at the same time, which was reversed, which further proved the inhibitory effect of mir-181a-5p on the motility of esophageal cancer. This evidence concerns the gene MAP2K1 and esophageal cancer.