In control testes expressing the G-TRACE cassette alone (E132-Gal4, Gal80ts > G-TRACE), hub cell nuclei were marked with both RFP (real-time Gal4 expression) and GFP (permanent marking expression), but cyst lineage cells outside the hub remained unmarked, as expected (Table 3; Figure 2A). This evidence concerns the gene LGALS4 and cyst.