LGALS4 and cyst: We shifted control (C587-Gal4, Gal80ts) and experimental (C587-Gal4, Gal80ts > UAS grim) flies to restrictive temperature (31 °C) for 2 days to ablate all early cyst lineage cells in the experimental flies, and then returned them to permissive temperature (18 °C) for 2 days.