Knockdown of PTEN in adult hub cells is also sufficient to cause their conversion to cyst lineage cells: after expressing PTEN RNAi together with G-TRACE in adult hub cells for 7 days (E132-Gal4, Gal80ts > UAS-Pten-RNAi + G-TRACE), we found a significant number of testes with GFP-marked cells outside the hub (Table 3; Figure 3F). Here, PTEN is linked to cyst.