To assess if the effect of PPE51 on phagocytosis of mycobacteria was TLR dependent, immortalized bone marrow-derived macrophage cell lines derived from either wild-type mice or gene knockout mice lacking expression of TLR2, TLR4, or TLR9 were infected with Msm expressing empty vector (Msm::EV) or PPE51 (Msm::51) at multiplicities of infection (MOI) of 5 and 10 for 3 h. The gene discussed is TLR9; the disease is infection.