Duplex miRNA mimics of miR-193-5p, miR-204, miR-29c, miR-30a, miR-30c, miR-32, miR-375, miR-532-3p, and miR-698 were injected into zygotes fertilized by naive sperm recapitulated the offspring stress dysregulation phenotype and induced long-term reprogramming of the hypothalamic transcriptome with HPA axis dysfunction.In miRNA injected embryos, target mRNAs Sirt1, Ube3a, IL6st, Ncl, Aars, Agfg1, and Ralbp1 were significantly reduced. Ube3a and Sirt1 are involved in chromatin remodeling and neurodevelopmental disorders. The gene discussed is AGFG1; the disease is neurodevelopmental disorder.