Moreover, high-dose Lactobacillus and Bifidobacterium probiotic supplements significantly alleviated the concentrations of TNF-α, insulin, and LDL-C (P = 0.009, P = 0.011, and P = 0.007, respectively) and the value of HOMA-IR in GDM rats (P = 0.000). This evidence concerns the gene INS and gestational diabetes.