Compared to the normal pregnant rats, the concentrations of TNF-α, NO, insulin, LDL-C, TC, and TG (P = 0.002, P = 0.003, P = 0.041, P = 0.000, P = 0.000, and P = 0.000, respectively) and the value of HOMA-IR were increased in GDM rats (P = 0.000). This evidence concerns the gene TNF and gestational diabetes.