After 3 weeks of injecting, TAK-242 (TLR4 inhibitor, 20 μg/mouse), PDTC (NF-κB inhibitor, 60 μg/mouse), or YC-1 (HIF-1α inhibitor, 20 μg/mouse) were injected into the subcutaneous tumor basement, and, after an hour, LPS (5 μg/mouse) was injected by the same method. The gene discussed is HIF1A; the disease is neoplasm.