Herein, we initially identified a set of 12 surface molecules, including CD39, CD229, CD81, CD326, CD54, CD130, CD49d, CD172a, CD274, CD155, CD205, and CD47 that are highly expressed by ASCs, and specifically identify ASCs in naive WT mice as well as in the contexts of immunization and autoimmunity. Here, CD274 is linked to Autoimmunity.