Together, our flow cytometry analysis demonstrates that CCR5 and CXCR6 are expressed by large proportions of all glioblastoma-infiltrating CD4+ T-cell subsets, whereas CCR2 and CXCR3 enrichment was only observed in glioblastoma-infiltrating CD4+ Tcm and Tregs, and Tem and Tregs respectively. This evidence concerns the gene CD4 and glioblastoma.