In addition, RNA-seq analysis of lncRNA CR46018-overexpressing Drosophila after infection with M. luteus revealed that upregulated genes were mainly enriched in Toll and Imd signaling pathways, supported by bioinformatics predictions and RNA-immunoprecipitation experiments which showed that CR46018 interacted with the transcription factors Dif and dorsal to enhance the Toll pathway. Here, TLR4 is linked to infection.