In a model of ischemia–reperfusion injury, the expression of NLRP3 and the activity of the inflammasome in the heart were low within 3 h after acute MI (AMI), significantly increased in the myocardium within 3–24 h, and peaked after 1 and 3 days in mice with reperfused and non-reperfused AMI, respectively, aggravating inflammatory response and the ischemia–reperfusion injury (16, 18, 20, 21, 25). The gene discussed is NLRP3; the disease is ischemia.