By analyzing RNA-seq data, we found five pathways that were both aberrantly altered in the DM1 group compared with the control group and significantly changed with miR-322/-503 treatment, which were TNF signaling pathway, Olfactory transduction, Steroid hormone biosynthesis, Linoleic acid metabolism, and Endocrine resistance. This evidence concerns the gene TNF and myotonic dystrophy type 1.