Second, for the quantitative monitoring for the overview of endotoxemia development, inflammatory factors such as TNF-alpha, IL-6, IL-8, IL-4, and some leukocyte antigens, such as macrophage-1 antigen (25) expression at different time points such as 0, 6, 10 and 24 h after LPS injection could be measured. The gene discussed is TNF; the disease is serum lipopolysaccharide activity.