In melanoma, CRISPR/Cas9 or lentivirus short hairpin RNA-mediated knockout or knockdown of the SIRT6 gene in A375 melanoma cells, leding to significantly reduced growth, vitality, and clonogenic survival rates of cancer cells, induced cell cycle arrest in G1 phase, and increased senescence-associated β-galactosidase staining (98, 99), reflecting the oncogenic activity of SIRT6. The gene discussed is SIRT6; the disease is cancer.