Moreover, western blot analysis revealed dramatically decreased levels of pyroptosis-associated proteins ASC, cleaved CASP1, GSDMD, cleaved GSDMD, cleaved IL-1β and cleaved IL-18 in the Ad-NLRP3-shRNA group compared with the Ad-GFP-shRNA group during fungal infection (Figures 4B,C). This evidence concerns the gene CASP1 and fungal infectious disease.