ANXA5 and Miyoshi myopathy: Since apoptosis and cell cycle are believed to be two of the main processes that contribute to cell survival, we subsequently incubated MM cell lines with increasing concentrations of SHP2 inhibitors for 48h and then used dual staining with Annexin V-APC/7-AAD and BrdU incorporation assay by flow cytometry to examine the apoptosis-inducing and cell cycle–inhibiting effects of SHP2 inhibitors in MM cells.