Overall methylation of SCNN1B gene in primary CF HBE (Fig. S5) resulted to be lower than that of CFBE (Figure S4), with a greatest responsiveness of primary CF HBE to SAM treatment that, in these cells, induced a greater hypermethylation (to 23.3%) than in CFBE (to 11.2%). Here, SCNN1B is linked to cystic fibrosis.