In the training cohort (n = 150), a high expression level of Piezo1 was closely correlated with tumor size, tumor nodular number, capsulation formation, high Edmondson–Steiner grade, micro- and macro-vascular invasion, advanced tumor node metastasis stage (TNM), Barcelona Clinic Liver Cancer (BCLC) stage and China Clinic Liver Cancer (CNLC) stage [2] (all P < 0.05, Table 1), and verified by Validation cohort. This evidence concerns the gene PIEZO1 and cancer.