CD274 and Familial prostate cancer: To test the effect of IFNγ on MHC-I, PD-L1 and E-cadherin expression in mCRPC cells, we evaluated one normal prostate cell line (RWPE) as well as different prostate cancer cell lines representing both androgen-dependent (LnCaP) and androgen-independent (DU145, PC3) subtypes of metastatic prostate cancer for their response to IFNγ (5 ng/mL, 48 h treatment).