As a result, intracellular ROS (reactive oxygen species) content decreased; also, the expressions of melanocortin 1 receptor (MC1R), MITF, tyrosinase, tyrosinase-related protein-1 (TRP1), and tyrosinase-related protein-2 (TRP2) were significantly decreased and melanogenesis was inhibited, although no cytotoxic effect was noticed upon the treatment of B16F10 melanoma cells. This evidence concerns the gene MC1R and melanoma.