To determine whether oMG are susceptible to HIV infection, we isolated microglia from 3D cerebral organoids generated from two iPSC lines (OH1.5 and OH3.1) and infected them with 10 ng (p24 Gag) of CCR5 M-tropic HIV-1 HxB2Balgp160 Luciferase reporter virus (HIVbal). Here, CCR5 is linked to HIV infectious disease.