ACBD3 and infection: For the assessment of the effect of protein 3A-C38 on ACBD3 distribution during infection, extracts of cells infected with CVB3 3A wild-type (wt) or CVB3 3A[C38A] virus were subjected to differential centrifugation to obtain a medium-speed membrane pellet (16 k), a high-speed membrane pellet (120 k), and a supernatant fraction representing the cytosolic fraction.