LTF and infection: We thus inoculated HepG2-NTCP cells with a mixture of HBV and these three types of lactoferrin at various concentrations (0.04, 0.2, 1 mg/mL) for 24 h, and evaluated the HBV infectivity by measuring the levels of HBeAg and HBV DNA in the supernatants of cell cultures at 7 days after infection.