In this review, which is a follow-up to the manuscript published in October 2021 [40], we present some more, less known biomolecules, i.e., CMPF, ANGPTL-8, nesfatin-1, afamin, adropin, fetuin-A, zonulin, SFRPs, and amylin, which could extend the list of early predictors and help to better plan the strategy of maternity care, thereby reducing the risk of negative consequences and complications caused by GDM. Here, NUCB2 is linked to gestational diabetes.