We found increased levels of phosphorylated Akt and JNK in Spp1-treated Pr111 PIN cells in comparison to control cells, whereas the levels of activated ERK1/2 and phospho-p38 MAPK remained similar or slightly reduced in Pr111 PIN cells when treated with recombinant Spp1. This evidence concerns the gene SPP1 and prostate intraepithelial neoplasia.