To test whether activated Akt and/or JNK signaling contributes to macrophage Spp1-induced PIN cell proliferation, we simultaneously added a JNK-specific inhibitor, SP600125, and/or an Akt-specific inhibitor, Wortmannin, to Pr111 PIN cells that were treated with macrophage Raw 264.7-conditioned media in a 3D setting. This evidence concerns the gene AKT1 and prostate intraepithelial neoplasia.