Therefore, we employed the ‘maftools’ package to analyze the somatic mutation profile between the high- and low-GNG4 groups in the TCGA-BLCA cohort; the top 10 most commonly mutated genes in each group are shown in Figure 2A. Previously published studies have claimed that genetic variations in the tumor suppressor gene TP53 contribute to human cancers in different ways, whereas TTN mutation was associated with responses to immunotherapy in solid tumors [35,36]. This evidence concerns the gene GNG4 and cancer.