In summary, miR-129-5p can induce apoptosis and inhibit the proliferation of DPCs, and participate in maintaining skin development, the HF cycle, and hair growth balance by targeting HOXC13. These results help to further understand the role and mechanism of miR-129-5p in the periodic development of hair follicles, which not only provides a theoretical basis for genetically improving the quality of hair in animals in the future, but also a new theory and method for diagnosing and treating hair loss in humans. Here, HOXC13 is linked to alopecia.