Finally, NOTCH3 transcript levels were increased in all MPS types but MPS II, MPS IVA, and MPS VI, whereas elevated amounts of the NOTCH3 protein were detected only in MPS I and MPS II, in contrast to a significant decrease observed in MPS IIID, MPS IVB, MPS VII, and MPS IX, also pointing to the partial accordance between the two methods of the estimation of gene expression efficiency (Table 2 and Figure 4). The gene discussed is NOTCH3; the disease is mucopolysaccharidosis.