In a comparison of marker triplets, AFP-HMMR-PITX, HMMR-NXPH4-PITX1 and HMMR-PITX1-THBS4, all exhibited better AUC, sensitivity, specificity, accuracy and other values in patients with HCC or eHCC compared with non-tumor cases. The gene discussed is PITX1; the disease is hepatocellular carcinoma.