Staining of immune cell surface markers was performed in all 43 esophageal adenocarcinoma specimens, including T lymphocytes (CD3+), B lymphocytes (CD20+), T cytotoxic cells (CD8+), T helper cells (CD4+), T memory cells (CD45RO+), T regulatory cells (FOXP3+), macrophages (CD68+), and M2 macrophages (CD163+) (Figure 3). The gene discussed is CD4; the disease is esophageal adenocarcinoma.