CD4 and neoplasm: Furthermore, strong positive correlations were found between levels of FoxP3+Helios− Tregs with CD4+TIM-3+ T cells and CD4+LAG-3+ T cells (r = 0.636, p = 0.002; r = 0.502, p = 0.017, respectively) in tumor tissues but not in PBMCs and NILs (Figure 4B,C).