Moreover, strong positive correlations were observed between levels of FoxP3+Helios+ Tregs with CD4+TIM-3+ T cells and CD4+LAG-3+ T cells (r = 0.767, p < 0.0001; r = 0.749, p < 0.0001, respectively) in tumor tissues but not in circulation or normal tissues (Figure 3B,C). This evidence concerns the gene CD4 and neoplasm.