The strongest gene–gene interactions contributing to the risk of ischemic stroke were found between SNP rs2301022 of the GCLM gene and two GWAS loci, such as rs4322086 of RASEF and rs12449964 of PEMT (exact biological functions of these genes remain unknown), suggesting that the studied genes are part of a complex genetic predisposition to ischemic stroke. Here, RASEF is linked to ischemic stroke.