Silencing of TTYH1 inhibited the migration and invasion of the U2OS osteosarcoma cells and reduced the expression of epithelial-mesenchymal transition (EMT)-related factors such as SNAIL, Zinc E-Box Binding Homeobox 1 (ZEB1), matrix metalloproteinase 2 (MMP2), MMP9, and N-cadherin. Here, MMP2 is linked to osteosarcoma.