To explore whether TTYH1 expression is involved in the progression of osteosarcoma, we investigated the endogenous expression of TTYH1 at mRNA and protein levels using RT-PCR and Western blotting, respectively, in three different human osteosarcoma cell lines (U2OS, SaSO2, and MG-63). This evidence concerns the gene TTYH1 and osteosarcoma.