What the EVs derived from the fusion-negative RMS cell lines had in common was the enrichment of miRNA related to cancer progression, including Cyclin proteins (Cyclin D1, IGF, AKT, SP1, and CDKN2A), and proteins involved in cell proliferation (NFYB), invasion (YBX1), and cell transformation (HMGA1) [70]. The gene discussed is YBX1; the disease is cancer.