In addition to i-SOX2-LNCaP and i-SOX2-ONS76, characterization of three additional engineered i-SOX2 tumor cell lines indicated that elevating SOX2 decreases MYC mRNA and protein in five tumor cell lines representing three human cancer types (prostate, medulloblastoma, and colorectal). The gene discussed is MYC; the disease is medulloblastoma.