In order to analyze whether HCC cells that express only the CSC marker CD90 are sensitive for AKT and mTOR inhibitors, we used the HCC-related cell line SK-HEP1 from ascitic fluid that highly expresses CD90 but no other CSC markers, i.e., EpCAM, AFP or CD133 [26]. Here, AKT1 is linked to hepatocellular carcinoma.