To determine the underlying mechanism by which the dietary VA and fat statuses regulate glucose and fat metabolism, we measured the hepatic mRNA levels of Gck, Srebp-1c, Srebp2, Fas, Acl, and Pck1 in ZL and ZDF rats fed a VAD, VAM or VAS diet with BF or HF content. Here, SREBF2 is linked to hydrops fetalis.