A subsequent study evaluating the 12 most frequently mutated genes confirmed an increased mutational load in different stages of monoclonal gammopathies: 21% in IgM-MGUS (additional mutations in at least 1/12 genes), 35% in aWM (by 8/12 genes) and 50% in symptomatic WM (by 12/12 genes) [93]. This evidence concerns the gene CD40LG and monoclonal gammopathy.