HSPB1 and colonic neoplasm: Additionally, a cytoprotective effect of Hsp27 against doxorubicin, via an MDR-independent mechanism, was also observed by another in vitro study, in which ten thermoresistant Chinese hamster cell lines were transfected with a plasmid containing the whole gene of human Hsp27 [77] as well as in a study in which HT-29 human colon cancer cell lines were transfected with the Hsp27 gene [78].