To investigate CPD0857 activity in colon cancer lines, we examined and quantified CD44 and CD133 expressions in various colon cancer lines, such as CCD-18Co, HCT15, HT-29, (Caco2, SW480, SW480APC15, HCT116 (HAE6) MT, HCT116 HAE6) WT, D-K-Ras WT, SW480APC38, HCT116 and D-K-Ras MT (Figure 4A). The gene discussed is PROM1; the disease is malignant colon neoplasm.