While using the AdnaTest BreastCancer system, one sample was considered CTC positive if at least one PCR fragment of one of the tumor-associated transcripts (MUC1, HER2, and GA733-2) and a fragment of the internal PCR control gene (gene β-actin) were detected; with the CellSearch system, a sample was considered positive if there were ≥5 CTCs detected in 7.5 mL of blood. This evidence concerns the gene ACTB and neoplasm.