MTAP and glioblastoma: To further test the effect of TMZ and ALA combination treatment in vivo, we orthotopically implanted MTAP-deficient GBM cells expressing luciferase into athymic nude mice, randomized the mice into four groups of treatment: vehicle (saline), TMZ (5 mg/kg), ALA (150 mg/kg, a dose that was 33% lower than that was used in our previous study [12]), and TMZ + ALA (TMZ 5 mg/kg + ALA 150 mg/kg), and monitored tumor progression by weekly bioluminescent IVIS imaging (Figure S5E).