The biological effects of Nigella sativa L. are attributed to the various experimental studies on animal models have proven that it acts as the immunomodulator in autoimmune diseases by reducing cluster of differentiation (CD)8+ and increasing the percentage of CD4+ CD25+ T cells in rheumatoid arthritis (RA) patients (Kheirouri et al., 2016). This evidence concerns the gene CD8A and rheumatoid arthritis.