The risk of major amputation was, for example, higher in men, people with neuropathy, foot ulcers with higher Wagner score, patients with worse diabetes control, with higher leucocytes, thrombocytes, C-reactive protein, and decrease in HDL-cholesterol, albumin, C-peptide, uric acid, and ankle-brachial index below 0.8 [26–28]. This evidence concerns the gene CRP and diabetes mellitus.