Additionally, immunohistochemistry analysis regarding the expression of CD31 (endothelial cell proliferation marker), VEGF (dominant melanoma angiogenesis inducer), and F4/80 (pan macrophage marker related to TAMs-induced angiogenesis) (Miettinen et al., 1994; Banciu et al., 2008b; Ge and Ding, 2020) was performed to validate the antiangiogenic activity of IL-13-LCL-SIM and PEG-EV-DOX. This evidence concerns the gene VEGFA and melanoma.