As HIF-1 is known to play a direct role in VEGF-mediated angiogenesis (Malekan et al., 2021) and several of our studies have reported strong inhibitory action of SIM on this important transcriptional regulator in tumor cells (Licarete et al., 2017; Rauca et al., 2018; Rauca et al., 2021), we investigated by WB whether our novel sequential therapy induced a similar effect. This evidence concerns the gene HIF1A and neoplasm.