Figure 4 shows that in comparison to the siRNA NC group, the migration ability in siRNA-USP25 transfected U251 cells or U87 cells was significantly decreased (P < 0.05). On the contrary, compared with OE NC, the migration ability was significantly increased after USP25 overexpression (P < 0.05), indicating that glioma cell migration ability was greatly facilitated by USP25. Here, USP25 is linked to central nervous system cancer.