To establish a clinical diagnostics study on CNS leukemia with state-of-the-art candidate subcellular biomarkers, we analyzed serial liquid biopsies from the CNS and bone marrow (BM) niches in children with ALL by measuring VEGF-A and ITGA6 concentrations and correlating these data with miR-181a expressions. This evidence concerns the gene ITGA6 and acute lymphoblastic leukemia.