To fully understand the effect of HtrA2/Omi on hepatic steatosis and related metabolic disorders, we further set up hepatic HtrA2/Omi-overexpressed mice models through tail-vein injections of AAV8-TBG-mNeongreen-HtrA2/Omi (Fig. 2A). This evidence concerns the gene HTRA2 and Hepatic steatosis.