To further investigate whether USP22 is involved in the regulation of fatty acid degradation, we examined the expression of fatty acid degradation-related enzymes (CPT1A, CPT2, ACOX1, ACADL, and ECHS1) in these USP22-engineereed stable cells by using qRT-PCR and western blot, and found that USP22 did not affect the expression of these enzymes in HCC cells (Supplementary Fig. 5d–f). This evidence concerns the gene ACADL and hepatocellular carcinoma.