To further investigate whether USP22 is involved in the regulation of fatty acid degradation, we examined the expression of fatty acid degradation-related enzymes (CPT1A, CPT2, ACOX1, ACADL, and ECHS1) in these USP22-engineereed stable cells by using qRT-PCR and western blot, and found that USP22 did not affect the expression of these enzymes in HCC cells (Supplementary Fig. 5d–f). Here, ECHS1 is linked to hepatocellular carcinoma.