The present study also demonstrated a significant association between the E-cad expression and tumor differentiation as the percentage of cases exhibiting abnormal expression of E-cad was significantly higher in poorly/undifferentiated carcinomas (96.0%) than in well-differentiated carcinomas (62.5%; p = 0.0115), in accordance with a previous report in canine colorectal carcinomas [70] and other studies in human GCs [29,54]. This evidence concerns the gene CDH1 and neoplasm.